Jose Manuel Guisán
Jose Manuel Guisán
Profesor de Investigacion
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Improvement of enzyme activity, stability and selectivity via immobilization techniques
C Mateo, JM Palomo, G Fernandez-Lorente, JM Guisan, ...
Enzyme and microbial technology 40 (6), 1451-1463, 2007
Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes
JM Guisán
Enzyme and Microbial Technology 10 (6), 375-382, 1988
Immobilization of enzymes and cells
JM Guisan
Humana Press, 2006
A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports
A Bastida, P Sabuquillo, P Armisen, R Fernández‐Lafuente, J Huguet, ...
Biotechnology and bioengineering 58 (5), 486-493, 1998
Immobilization of lipases by selective adsorption on hydrophobic supports
R Fernandez-Lafuente, P Armisén, P Sabuquillo, G Fernández-Lorente, ...
Chemistry and physics of lipids 93 (1-2), 185-197, 1998
Different mechanisms of protein immobilization on glutaraldehyde activated supports: effect of support activation and immobilization conditions
L Betancor, F López-Gallego, A Hidalgo, N Alonso-Morales, GDOC Mateo, ...
Enzyme and microbial technology 39 (4), 877-882, 2006
Interfacial adsorption of lipases on very hydrophobic support (octadecyl–Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases
JM Palomo, G Muñoz, G Fernández-Lorente, C Mateo, ...
Journal of Molecular Catalysis B: Enzymatic 19, 279-286, 2002
Glyoxyl agarose: a fully inert and hydrophilic support for immobilization and high stabilization of proteins
C Mateo, JM Palomo, M Fuentes, L Betancor, V Grazu, F López-Gallego, ...
Enzyme and Microbial Technology 39 (2), 274-280, 2006
Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment☆
C Mateo, O Abian, R Fernandez–Lafuente, JM Guisan
Enzyme and Microbial Technology 26 (7), 509-515, 2000
Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment
C Mateo, O Abian, G Fernández‐Lorente, J Pedroche, ...
Biotechnology progress 18 (3), 629-634, 2002
Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their …
C Mateo, G Fernández-Lorente, O Abian, R Fernández-Lafuente, ...
Biomacromolecules 1 (4), 739-745, 2000
Enzyme stabilization by glutaraldehyde crosslinking of adsorbed proteins on aminated supports
F López-Gallego, L Betancor, C Mateo, A Hidalgo, N Alonso-Morales, ...
Journal of Biotechnology 119 (1), 70-75, 2005
Some special features of glyoxyl supports to immobilize proteins
C Mateo, O Abian, M Bernedo, E Cuenca, M Fuentes, ...
Enzyme and Microbial Technology 37 (4), 456-462, 2005
Immobilization of enzymes on heterofunctional epoxy supports
C Mateo, V Grazu, JM Palomo, F Lopez-Gallego, R Fernandez-Lafuente, ...
Nature Protocols 2 (5), 1022-1033, 2007
Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method
R Fernandez-Lafuente, CM Rosell, V Rodríguez, C Santana, G Soler, ...
Enzyme and Microbial Technology 15 (7), 546-550, 1993
Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support–polyethylenimine composites
C Mateo, O Abian, R Fernandez‐Lafuente, JM Guisan
Biotechnology and Bioengineering 68 (1), 98-105, 2000
Epoxy-amino groups: a new tool for improved immobilization of proteins by the epoxy method
C Mateo, R Torres, G Fernández-Lorente, C Ortiz, M Fuentes, A Hidalgo, ...
Biomacromolecules 4 (3), 772-777, 2003
General trend of lipase to self-assemble giving bimolecular aggregates greatly modifies the enzyme functionality
JM Palomo, M Fuentes, G Fernández-Lorente, C Mateo, JM Guisan, ...
Biomacromolecules 4 (1), 1-6, 2003
Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: correlation between enzyme …
J Pedroche, M del Mar Yust, C Mateo, R Fernández-Lafuente, ...
Enzyme and Microbial Technology 40 (5), 1160-1166, 2007
Immobilization-stabilization of enzymes; variables that control the intensity of the trypsin (amine)-agarose (aldehyde) multipoint attachment
RM Blanco, JJ Calvete, JM Guisán
Enzyme and microbial technology 11 (6), 353-359, 1989
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